Skip to main content
. 2015 Dec 11;9(12):e0004273. doi: 10.1371/journal.pntd.0004273

Fig 4. Sensitivity and specificity of the nested PCR for Leishmania minicircle kDNA.

Fig 4

Nested PCR amplification products from serial dilution curves of DNA from L. V. panamensis promastigotes (A) and human PBMCs (B). The upper panel shows the first amplification product of 700 bp obtained with LVp1 primer sets (external primers) and the bottom panel shows the 180 bp product of the nested PCR obtained with LVp1-LVp5 primer sets (internal primers). Nested PCR amplification products from reference strains of L.V. panamensis (L.p), L.V. guyanensis (L.g), L.V. braziliensis (L.b), L. infantum (L. inf), L. amazonensis (L.ama), L. mexicana (L. mex) and T. cruzi (C). C-, negative control—water; PBMCs, DNA from uninfected human PBMCs.