Table 1. Summary of targeted mutation screen of primary events and enrichment PCR results from callus.

Diploid (X914-10) and tetraploid (Désirée) genotypes were stably transformed with gRNA746 and gRNA751 CRISPR/Cas reagents in a conventional 35S or geminivirus LSL T-DNA backbone using hygromycin selection (Total events). A restriction enzyme digestion assay and quantification of resistant and digested bands were used to identify events with at least 1% mutation frequencies (# with mutations) and events above a threshold using expected single allele mutation frequencies (# above threshold) (S2 Table). Percentages are of total events and modified enrichment PCR results come from Fig 1B and S1 Table.

Genotype	gRNA	T-DNA	Total events	# with mutations	% with mutations	# above threshold	% above threshold	Modified Enrichment PCR
X914-10	746	35S	27	15	55%	4	15%	+
X914-10	746	LSL	32	13	41%	1	3%	-
X914-10	751	35S	35	3	9%	1	3%	+
X914-10	751	LSL	39	1	3%	0	0%	-
Désirée	746	35S	35	21	60%	10	29%	+
Désirée	746	LSL	33	12	36%	0	0%	-
Désirée	751	35S	37	4	11%	1	3%	+
Désirée	751	LSL	21	1	5%	0	0%	-