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. 2015 Dec 14;10(12):e0144674. doi: 10.1371/journal.pone.0144674

Fig 2. Effect of root RNAi-mediated silencing nematode genes on development and reproduction of Pratylenchus penetrans.

Fig 2

(A) Semi-quantitative RT-PCR expression of Pp-pat-10 and Pp-unc-87 from mRNA of differential nematode stages (eggs, juveniles and adult stages). (B) Nematode challenge assays in transgenic soybean dsRNA hairy root lines 3 months after infection. Data shown represent the total mean number ± SEM of nematodes recovered from roots, using a pool of nine soybean hairy roots for each line. Replicate 1 and replicate 2 correspond to two independent biological assays, using the same hairy root lines. As control, hairy roots transformed with an empty vector (pBinJIT) were used. Asterisks denote statistically significant differences using Student’s t- test (P < 0.05). (C) Transcript levels of P. penetrans targeting genes that develop in the dsRNA expressing transgenic soybean hairy root lines. The expression levels were quantified as fold change values calculated by the 2-ΔΔCt method, using 18S rRNA gene as reference. Each bar represents the mean with standard error of two independent biological replicates, using three technical repetitions. Asterisks denote statistically significant differences using Student’s t-test (P < 0.05).