Table 1. Overview of sequencing and de novo assembly results of mRNA-Seq analyses using RNA collected from Pratylenchus penetrans.

Sample	Total Bases	Read Count	GC (%)	Q20 (%)	Q30 (%)
Pool of nematodes (sample 1)	7,916,194,968	78,378,168	43.21	92.18	87.37
Pool of nematodes (sample 2)	7,202,319,696	71,310,096	43.84	93.56	87.65
	Trinity assembly K = 25
	n	n: 100	n: N50	min	median	mean	N50	max
de novo assembly	23,715	23,715	5,133	201	578	882	1,362	8,605

Only sequences >200 bp were considered for this analysis.