Table 1. List of genes of RAW 264.7 cells activated with LPS (500 ng/mL) which were regulated by the treatment with 50 μg BRP/mL.
Fold changes were calculated in relation to LPS activated cells with and treated with DMSO (control). Experiments performed in triplicate.
| Gene | Fold-change | P value | Function |
|---|---|---|---|
| Ccnd1 | -3.1 | 0.019304 | PI3K/AKT pathway [27] |
| Cd14 | -2.1 | 0.00953 | TLR response [28] |
| Cd80 | 4.7 | 0.03 | Macrophage polarization marker [8, 29] |
| Cd86 | 4.3 | 0.02 | Macrophage polarization marker[8, 29] |
| Eif2ak2 | -2.3 | 0.006488 | NF-κB pathway [30] |
| Elk1 | -7.3 | 0.000049 | TLR response [31] and cancer development [32] |
| Flt3l | -4.1 | 0.00104 | PI3K/AKT pathway [33] |
| Fos | -5.9 | 0.00083 | Cancer development [34] |
| Gdf1 | -2.7 | 7E-06 | TGF-beta super-family [35] |
| Il10 | -6.0 | 0.03872 | Anti-inflammatory cytokine [36]. |
| Il1b | -6.2 | 0.050638 | Inflammatory cytokine [37, 38] |
| Il1f9 | -27.5 | 0.03639 | Inflammatory cytokine [39] |
| Il1rn | -4.2 | 0.00048 | IL-1 pathway [40] |
| Map2k1 | -3.3 | 0.032279 | MAPK pathway [41] |
| Mapk14 | -4.1 | 0.014773 | MAPK pathway [42] |
| Mcr | -2.5 | 0.04 | Macrophage polarization marker[8, 29] |
| Mtcp1 | -7.9 | 0.000981 | Increase activation of AKT1 and AKT2 [43] |
| Naip1 | 6.3 | 0.032882 | Anti-apoptotic [44] |
| Nfkb1 | -5.8 | 0.05567 | NF-κB pathway [45] |
| Pak1 | -17.3 | 0.032134 | MAPK pathway [46] |
| Pdk1 | -5.6 | 0.031172 | NO pathway [47] |
| Pik3ca | -2.1 | 0.024495 | PI3K/AKT pathway[48] and involved in cancer [49] |
| Pik3cg | -2.7 | 0.008274 | PI3K/AKT pathway and TLR response [50] |
| Pik3r2 | -2.6 | 0.020004 | PI3K/AKT pathway [48] |
| Prkca | -4.7 | 0.000282 | MAPK pathway [51] |
| Rps6ka1 | -2.2 | 0.017273 | MAPK pathway [52] |
| Srf | -4.8 | 0.038967 | MAPK pathway [53] |
| Tirap | -47.9 | 0.022142 | TLR response [54] |
| Tlr4 | -3.6 | 0.018578 | TLR response [55] |
| Tnfsf12 | -2.6 | 0.00383 | TNF-α cascade [56] |