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. 2015 Dec 15;10(12):e0144840. doi: 10.1371/journal.pone.0144840

Fig 4. CREB is required for NTHi-induced MyD88 short expression in airway epithelial cells.

Fig 4

(A) BEAS-2B cells were stimulated with NTHi at various time intervals as indicated. Phospho-CREB and total CREB were visualized via western blot analysis. (B) Cells were transfected with the CRE-luciferase construct and stimulated with NTHi for 6 hours. Relative luciferase activity was measured. (C-E) Cells were (C-D) transfected with CREB siRNA or control siRNA or (E) treated with CBP-CREB inhibitor (100 μM) prior to NTHi stimulation for 6 hours. Relative quantity of human MyD88s mRNA was measured via real-time Q-PCR analysis. Data are mean ± SD (n = 3). *p<0.05. Statistical analysis was performed using Student’s t-test. Data are representative of three or more independent experiments.