Skip to main content
. 2015 Dec 15;10(12):e0144840. doi: 10.1371/journal.pone.0144840

Fig 5. ERK 1/2 is a negative regulator for NTHi-induced MyD88 short expression in airway epithelial cells.

Fig 5

(A) BEAS-2B cells were stimulated with NTHi at various time intervals as indicated. Phospho-ERK and total ERK were visualized via western blot analysis. (B) Cells were treated with PD98059 (10 μM) followed by NTHi stimulation for 6 hours. The relative quantity of human MyD88s was measured by real-time Q-PCR analysis. (C-H) Cells were transfected with (C) ERK1-DN, (D) ERK2-DN, (E-F) ERK1 siRNA or (G-H) ERK2 siRNA. Following NTHi stimulation for 6 hours, the relative quantity of MyD88s was measured by real-time Q-PCR analysis. Data are mean ± SD (n = 3). *p<0.05. Statistical analysis was performed using Student’s t-test. Data are representative of three or more independent experiments.