Table 1.

Primer sequences and conditions for RT-PCR

Target genes (Accession number)	Primer (Forward, Reverse)	Anneling Tm(°C)	PCR cycles
TRAP (NM_007388)	5′-ctgctgggcctacaaatcat-3′ 5′-ggtagtaagggctggggaag-3′	54	30
RANK (NM_009399)	5′-aaaccttggaccaactgcac-3′ 5′-accatcttctcctcccgagt-3′	53.7	30
MMP9 (NM_013599)	5′-cgtcgtgatccccacttact-3′ 5′-agagtactgcttgcccagga-3′	57.5	36
Cathepsin K NM_007802)	5′-aggcggctatatgaccactg-3′ 5′-ccgagccaagagagcatatc-3′	57.5	26
MMP2 (NM_008610)	5′-ggctggaacactaggac-3′ 5′-cgatgccatcaaagacaatg-3′	60	36
CAII (K00811)	5′ ttggctgttttgggctatttt-3′ 5′- cgctttgatctttctattctt -3′	52	35
DC-STAMP (AY517483)	5′- ctaaggagaagaaacccttg-3′ 5′- cagcatagaagacaacaatcc -3′	54	35
PU.1 (M_32370)	5′- cttcccttatcaaaccttgtc-3′ 5′- aggtgagcttcttcttgactt -3′	54	35
MITF (NM_008601)	5′- agacctgacatgtacgacaac-3′ 5′- tatgcagggctactgataag -3′	56	35
c-src (U01149)	5′- tccaggctgaggagtggtactttgg-3′ 5′- atacggtagtgaggcggtgacacag -3′	64	35
c-myc (AB040746)	5′- caccagcagcgactctgaagaagag-3′ 5′- agaggtgagcttgtgctcgtctgc -3′	64	35
GAB2 (AB018414)	5′- ctggacaagaaccacaatgc-3′ 5′- agtctttcctggaggttcag -3′	56	30
β-actin (NM_007393)	5′-ttctacaatgagctgcgtgt-3′ 5′-ctcatagctcttctccaggg-3′	50	26