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. Author manuscript; available in PMC: 2017 Jan 5.
Published in final edited form as: Mol Cell Endocrinol. 2015 Sep 30;419:29–43. doi: 10.1016/j.mce.2015.09.030

Figure 2. RT-qPCR validation of selected genes and comparison with microarray data.

Figure 2

qPCR analysis was performed on selected genes involved in fatty acid biosynthesis (A), cholesterol biosynthesis (B) and cholesterol efflux (C). qPCR results are presented as mean ± s.d. (n=3 pools of 30 larvae each), expression levels were normalized to β-actin (bac2) and statistical significance was determined using unpaired two-tailed Student’s t-test compared to the control treatment (*P<0.5,**P<0.01,***P<0.001,****P<0.0001). Black bars represent microarray results and white bars RT-qPCR results. T0901317 and GW3965 treatments are abbreviated as T0 and GW, respectively.