Figure 8. Regulation of genes involved in visual perception in juvenile zebrafish treated with GW.
1 month old juvenile zebrafish were treated with 1 μM GW or vehicle only (0.1% DMSO) daily for 4 days, and the mRNA content of the genes related to visual perception rx2, guca1d, guca1g, guca1b, grk7a, rgs9a and crygs2 was assessed by RT-qPCR (A). 1 month old juvenile zebrafish were treated with 1 μM GW or vehicle only (0.1% DMSO) daily for 4 days, and the mRNA level of genes containing the PCE-1 conserved element (rho, irbp, sagb) was assessed by RT-qPCR (B). Expression levels were normalized to β-actin (bac2) and each individual value is presented with the mean indicated by a line; Control (solid circles), n=9 and GW (solid squares), n=8. Statistical analysis was performed with unpaired two-tailed Student’s t-test.