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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Curr Opin Chem Biol. 2015 Oct 24;29:72–78. doi: 10.1016/j.cbpa.2015.10.001

Figure 3. Some sgRNAs allow binding or cleavage at variants of the target site.

Figure 3

In addition to single-base mismatches (a), some sgRNAs can tolerate DNA sequences with an extra base (b, DNA bulge) or a missing base (c, sgRNA bulge).