Fig 6. Effects of Y-27632 on the cell cycle distribution and apoptosis of human limbal epithelial cells.

Human limbal epithelial cells were expanded on plastic dishes with or without 20uM Y-27632 in culture medium at 37°C for 30 hours. After trypsinization, cells were fixed, stained with PI and analyzed by flow cytometry. Statistical analyses were performed using a paired test, and *p<0.05 was considered significant. The percentage of apoptotic cells (subG1 phase) was reduced from 2.9±0.9% (control) to 1.7±0.5% in the presence of Y-27632 (p = 0.24, n = 5). On the other hand, the percentage of proliferating cells (S-phase) was increased from 5.1±1.3% (control) to 7.8±1.9% in the presence of Y-27632 (p = 0.0295, n = 5).