A, HeLa cells were transfected with GFP-PAQR3 for 36–48 h and then treated with 10 μM gallein (bottom row) or vehicle (top row) for 8 h prior to being fixed and processed for immunofluorescence microscopy using anti-TGN46 to detect Golgi morphology. Expressed GFP-PAQR3 was detected by intrinsic GFP fluorescence. B, HeLa cells were transfected with GFP-PAQR3 together with GRK2ct. Cells were fixed and stained with anti-GRK2, and the intrinsic GFP fluorescence allowed visualization of GFP-PAQR3, and, by extension, Golgi morphology. Bar, 10 μm.