Skip to main content
. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cell Signal. 2015 Aug 29;27(12):2444–2451. doi: 10.1016/j.cellsig.2015.08.017

Figure 3. PKD inhibitors PKD-DN and Gö6976 inhibit PAQR3-mediated Golgi fragmentation.

Figure 3

A, HeLa cells were transfected with GFP-PAQR3 for 36–48 h and then treated with 5 μM Gö6976 (bottom row) or vehicle (top row) for 8 h prior to being fixed and processed for immunofluorescence microscopy using anti-TGN46 to detect Golgi morphology. Expressed GFP-PAQR3 was detected by intrinsic GFP fluorescence. B, HeLa cells were transfected with GFP-PAQR3 together with HA-PKD-DN (PKD-K618N). Cells were fixed and stained with anti-HA, and the intrinsic GFP fluorescence allowed visualization of GFP-PAQR3, and, by extension, Golgi morphology. Bar, 10 μm.