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Effect of LPS on levels of COX-2, proinflammatory molecules, and ALOX5 in ATII cells from WT and AT2CC−/− mice. ATII cells from WT and AT2CC−/− mice were isolated and treated with saline or LPS (1 µg/ml in 1% FBS/DMEM) for 4 h. ATII cells were collected, and mRNA levels of Cox-2 (A), Il-1b (B), Il-6 (C), and Alox5 (D) were determined by real-time RT-PCR (n = 4). *P < 0.05 vs. saline of same genotype; ^P < 0.05 vs. WT saline; #P < 0.05 vs. WT LPS.
