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Eicosanoid levels in ATII cells isolated from WT and AT2CC−/− mice. ATII cells from WT and AT2CC−/− mice were isolated and treated with saline or LPS (1 µg/ml in 1% FBS/DMEM) for 4 h. The media were collected, and eicosanoids were extracted with C18 columns and quantified by LC/MS/MS. Levels of 6-keto-PGF1α (A), TXB2 (B), PGF2α (C), PGD2 (D), PGE2 (E), LTB4 (F), and 20-COOH-LTB4 (G) were detected (n = 4). *P < 0.05 vs. saline of same genotype; #P < 0.05 vs. WT.
