Figure 6.

COX expression and eicosanoid levels in LPS-treated WT and AT2CC^−/− mice. WT and AT2CC^−/− mice were treated with saline or LPS (50 μg via oropharyngeal aspiration) for 4 h. COX-1 (A) and COX-2 (B) levels were determined in lung tissues by real time RT PCR. BALF levels of PGE₂ (C) and PGD₂ (D) were measured by LC/MS/MS. PTGDS (E) and PTGES (F) levels were determined in lung tissues by real time RT PCR. BALF levels of LTB₄(G) and 20-COOH-LTB₄ (H) were measured by LC/MS/MS. I) ALOX5 mRNA levels were determined in lung tissues by real time RT PCR (n = 12–31). *P < 0.05 vs. saline of same genotype; ^P < 0.05 vs. WT saline; ^#P < 0.05 vs. WT LPS.