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Bleomycin-induced inflammation in WT and AT2CC−/− mice. WT and AT2CC−/− mice were treated with saline or bleomycin (1 mg/kg body weight) via oropharyngeal aspiration and studied 21 d later. Whole lungs were lysed, and mRNA levels of CCL2 (A), IL-1β (B), IL-6 (C), and TNF-α (D) were determined by real-time RT-PCR. Levels of IL-6 (E), and CCL2 (F) were measured in BALF (n = 8–10); *P < 0.05 vs. saline of same genotype.
