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Colocalization of Nrf2 with mitochondria. Neonatal rat cardiomyocytes were grown on glass cover slips. Following 300 μM H2O2 treatment for 2 h and 24 h of recovery, cells were fixed with formalin after 30 min of MitoTracker Deep Red uptake. Nrf2 was detected by immunocytochemistry as described in Materials and Methods.
