Figure 12.

Loss of Nrf2 increases mitochondrial sensitivity to calcium-induced swelling. Mitochondria were isolated from the hearts of WT or Nrf2 KO mice and challenged with calcium to induce mitochondria swelling. Swelling was monitored by a decrease in absorbance at 520 nm over a 20 min period at 37°C after addition of PBS (control) or calcium (A). Decrease in absorbance (%) following addition of calcium was calculated compared with WT or Nrf2 KO controls (B). Values represent the means ± sem from 3 independent experiments. The letter a indicates significant difference (P < 0.05) from means labeled with a letter b or c as determined by 1-way ANOVA with Bonferroni correction.