Figure 5.

Preconditioning with H₂O₂ protects against the decreases of cytochrome c and cytochrome b. Rat cardiomyocytes were pretreated with 100 μM H₂O₂ for 10 min followed by 1 h of recovery in fresh medium. Cells were collected at the time point indicated for measurements of Nrf2 protein (A, B). Cells with or without 10 min 100 μM H₂O₂ pretreatment were treated at 1 h after for varying concentrations of H₂O₂ for 2 h before harvesting 48 h later for Western blot analysis to measure cytochrome c and cytochrome b protein levels (C, D). The band intensity from Western blot was quantified using ImageJ software and normalized to the corresponding loading control VCL band for numeric presentation (B, D). The data are from 1 experiment representative of 3 (A, C) or means ± sd from 3 experiments (B, D).