Figure 7.

Nrf2 inhibits alteration of mitochondrial morphology following H₂O₂ exposure. Neonatal rat cardiomyocytes seeded on glass coverslips were infected with adenovirus for expression of GFP, Nrf2, or DN-Nrf2 (A). At 48 h after infection, cells were exposed 2 h to PBS (control) or 300 μM H₂O₂. Following 24 h of recovery, cells were stained with MitoTracker Deep Red 633 for observation under an Olympus BX53 fluorescent microscope with attached Olympus DP72. A view from Nrf2 adenovirus-infected cells treated with H₂O₂ showing the difference in mitochondrial morphology in the cells expressing Nrf2 vs. the cells not expressing Nrf2 (B).