Figure 9.

Nrf2 fails to reduce protein oxidation by H₂O₂ treatment. Neonatal rat cardiomyocytes were infected with adenovirus for expression of GFP, Nrf2, or DN-Nrf2. At 48 h after infection, cells were treated with 300 μM H₂O₂ for 2 h. Cells were harvested immediately for measurements of protein carbonyl. Values represent the means ± sd from 3 independent experiments. A letter a indicates significant difference (P < 0.05) from means labeled with b or c as determined by 1-way ANOVA with Bonferroni correction.