Table 1.
Stability of egg yolk powder IgY Ab titer to bovine RVA (Indiana G6P[5] strain) determined by ELISA in 10 mg/mL solution from samples kept at different storage conditions.
| Storage conditions | Days from egg yolk powder production |
||||||
|---|---|---|---|---|---|---|---|
| 0 | 30 | 90 | 180 | 360 | 540 | 720 | |
| 4 °C and room RH | 1024 | 1024 | 1024 | 1024 | 1024 | 1024 | 1024 |
| Room temperature and RH | 1024 | 1024 | 1024 | 1024 | 1024 | 1024 | 1024 |
| 30 °C — 65% RH | 1024 | 1024 | 1024 | 256 | 256 | 64 | 4 |
| 40 °C — 75% RH | 1024 | 1024 | 1024 | 256 | 256 | 4 | 4 |
After 0, 30, 90, 180, 360, 540 and 720 days from powder production, packages were opened and samples were prepared in milk as described in the Materials and Methods section. The IgY ELISA Ab titer to bovine RVA (Indiana G6P[5] strain) was determined immediately by ELISA assay. RH: relative humidity.