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. 2015 Dec 18;8:644. doi: 10.1186/s13071-015-1259-6

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© Marques et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression profiles of genes involved in immune response in the liver of wild-type mice chronically infected with different strains of L. infantum. The relative expression of Arg1 (a), Nos2 (b), Il-10 (c), Il-12p40 (d), Ifng (e) and Tnf (f) in the liver of wild-type mice after 60 days of infection with L. infantum ITMAP-263, IMT-151 or IMT-202 strains was measured by qRT-PCR. Each sample was normalized to Hprt, calculated by the comparative C_T method (2^-ΔΔCT). The expression was further normalized to expression levels in naïve mice’s liver. Mean and SD of each group are shown (N = 4–6). One-way ANOVA with post hocTukey test was performed (*p < 0.05; **p < 0.01; ***p < 0.001, versus non-infected control)