FIG. 5.

Western blot analysis. Western blotting for the HSP: HSP27, HSP70, and HSP90; osteogenic transcription factors: Smad1, Smad5, phospho-Smad1, phospho-Smad5, and OSX; and BMP2 and BMPR Type IA/II were performed using their respective polyclonal antibodies. The cultured cells were collected for Smad1, Smad5, phospho-Smad1, phospho-Smad5, OSX, and BMPR Type IA/II detection, and the culture medium was assayed for the HSPs and BMP2 secreted from the osteoblasts 24 h after the stimulation. Intensities of the developed bands were semiquantified by image analyzing software. Color images available online at www.liebertpub.com/tea