Table 1.
Properties of nine polymorphic tetra- and pentanucleotide microsatellite loci cloned from Bemisia tabaci biotype B
| Microsatellite locus name | Primer sequence (5′–3′ direction) | Repeat motif | Fluorescent dye color (Applied Biosystems) | MgCl 2 (mM) | Accession numbers | Biotypes (other than B) and haplotypes successfully cross-amplified |
|---|---|---|---|---|---|---|
| WF1B11 | F: GCATTGAACATTTTTCTGCATGCGCG | (CCTGA) 12 imp | NED | 1 | JQ436838 | A, H, K, L, M, Ms, Q, S, T, African, Asian, Australian, and New World haplotypes |
| R: GCACACAGCTCTCCAAAAGAAAGGTC | ||||||
| WF2C01 | F: ATGATACCGCACGAAAAAGAGGACG | (GTTT) 11 imp | NED | 1 | JQ436842 | L, M, Q, African, Asian, and New World haplotypes |
| R: CTTGAATTACATCAAACGCAGCAGC | ||||||
| WF2H06 | F: TATTCGCCAATCGATTCCTT | (TTTG) 11 | NED | 0.75 | JQ436844 | A, L, M, Ms, Q, African, New World, and Yemen haplotypes |
| R: CGGCGGAAATTTCGATAAA | ||||||
| WF1B06 | F: TCTCATAGAAAATTTTGGAGAAAGAAA | (ACTC) 8 | PET | 0.75 | JQ436837 | L, Ms, Q (only Spain Q or Q1), African, New World, and Yemen haplotypes |
| R: TGCGGAATCTCAGGGTCATA | ||||||
| WF2E11 | F: TCTCCAACCATAATTTTTAATCTCG | (GATT) 27 imp | PET | 1 | JQ436843 | L, M, Ms, Q, African, New World, and Yemen haplotypes |
| R: GTCTGGGCAGGAAAACGAT | ||||||
| WF1D04 | F: GTTGTTAGGTTACAGGGTTTGTC | (CAAA) 16 | 6-FAM | 1 | JQ436839 | A, S, T, African, and New World haplotypes |
| R: GTCTTTACTTCTCTTTTCCTCCG | ||||||
| WF1G03 | F: CTCCAAAATGGGACTTGAAC | (GTTT) 8 | NED | 1.125 | JQ436840 | H, K, M, Ms, Q, T, African, Asian, Australian, and New World haplotypes |
| R: GTAGAAGCCACACATACTAGCAC | ||||||
| WF2A05 | F: ATTGATCATTTTCGGCTACCTTATC | (CAAA) 12 imp | NED | 1.125 | JQ436841 | |
| R: CGACCCTTCTGTAAATGATAGACT | ||||||
| WF2A02 | F: GAAGTGCATAAAAACATCGTCGCCG | (CTTT) 10 imp | NED | 1 | JX853746 | |
| R: CAATTGAAAGGCTAGACTCCCTGGC |
Imp, imperfect.
Locus name, primer sequences, repeat motif, fluorescent dye color used in fragment analysis, MgCl 2 concentration, GenBank accession numbers, and successful cross-amplification in non-B biotypes and haplotypes.