Table 2.

Induced NGal-Ab after pig-to-baboon cardiac xenotransplantation.

Donor	Survival (days)	HEK-B4T IgM	HEK-B4T IgG	GalT-KO PAEC IgM	GalT-KO PAEC IgG
GalT-KO:CD55	71	−	+	0.5	0.6
GalT-KO:CD55	31	−	−	0.5	0.4
GalT-KO:CD55	28	+++	+++	4.8	2.5
GalT-KO:CD55	27	+++	+	2.2	8.5
GalT-KO	22	+++	++++	1.9	7.3
GalT-KO	21	−	−	1.3	0.9
GalT-KO:CD55	18	−	−	0.4	0.4

Antibody reactivity to HEK-B4T cells is estimated as the ratio of specific antibody binding to HEK-B4T relative to HEK cells and represented in a semi-quantitative scale with ratios less then 2 scored as negative, 2.0–2.5 +, 2.5–5.0 ++, 5.0–10.0 +++ and greater then 10 ++++. Antibody to GalT-KO PAECs is the fold increase in MFI in post explant serum compared to pretransplant serum. There is a positive and significant (p<0.05) Spearman rank correlation coefficient between the anti-PAEC and anti-HEK-B4T IgM (r = 0.8929) and IgG (r = 0.8571) immune response. Adapted from Byrne et al (35).