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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2015 Nov 4;89:690–700. doi: 10.1016/j.freeradbiomed.2015.08.028

Figure 5. Systemic administration of bixin attenuates UV-induced cutaneous hyperproliferation and inflammation in Nrf2+/+ mice but not Nrf2−/− mice.

Figure 5

Mice were treated as detailed in Figs. 3 and 4 followed by IHC analysis for (A) Ki67 and (B) MMP9 (scale bar: 100 μm). (C-D) Skin tissue lysates from bixin/UV-exposed Nrf2+/+ and Nrf2−/− mice were also subjected to immunoblot analyses with anti-p-p65, p65, and GADPH antibodies followed by quantification using densitometry (D). (E) mRNA levels of IL6, TNFa and MMP9 were determined using quantitative RT-PCR. Results are expressed as means ± SD (*p<0.05, control vs. treatment groups; #p<0.05, UV vs. bixin+UV groups).