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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2015 Nov 5;89:1184–1191. doi: 10.1016/j.freeradbiomed.2015.11.003

Figure 5.

Figure 5

Increased miR-146a with age reduces Nrf2 protein levels.

(A) Liver tissue from 6 young (6 months) and 6 old (24 months) rats were analyzed on a miRNA array. Results were limited to p values of less than 0.05 and corrected for multiple comparisons. The miRNAs predicted and/or experimentally determined to modulate Nrf2 are shown. (B) Liver tissue from 6 young and 6 old rats were analyzed by RT-qPCR for levels of miR-146a. The difference between young and old is significant by Student's t-test *p<0.05. (C) Hepatocytes isolated from young rats were transfected with rno-miR-146a miRNA mimic or scrambled RNA oligomer, treated with BTZ for 6 h, and compared to vehicle-treated hepatocytes (N=3 for each condition). Nrf2 protein levels and loading control Actin were measured by immunoblot analysis and quantitated by densitometry. Graph shows Nrf2 fold increase over non-BTZ-treated cells (= 1) (*p<0.05). (D) mRNA levels were measured and compared to hepatocytes transfected with a scrambled RNA oligomer (= 1.0). Although no significant difference was seen in the RQ of the Nrf2 mRNA, both Gclc and Gclm decrease significantly, *p<0.05.