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. Author manuscript; available in PMC: 2016 May 23.
Published in final edited form as: Nat Chem Biol. 2015 Nov 23;12(1):29–34. doi: 10.1038/nchembio.1966

Figure 4. Stability and structure of sTIM-11.

Figure 4

(a) Superposition of the X-ray crystal structure (in blue) and the design model (in pink). (b) Comparison of the crystal structure and design model over two internal repeat units (residues 47-92 and 93-138). The internal repeats are nearly identical, and their side chains are in perfect agreement with the model. (c) Chemical denaturation with guanidinium chloride (GdmCl) followed by CD (open circles) and fluorescence (closed circles). Signals for the secondary (CD) and tertiary (fluorescence) structures are lost simultaneously at ~2 M GdmCl. (d) Thermal melt followed by CD. (e) CD wavelength spectra of sTIM-11 at 30 °C (line), when melted at 95 °C (dashed), and after cooling at 30°C (dotted).