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. Author manuscript; available in PMC: 2016 Jun 18.
Published in final edited form as: ACS Chem Biol. 2015 Oct 15;10(12):2672–2679. doi: 10.1021/acschembio.5b00765

Figure 3.

Figure 3

Temperature sensitivity. Novel potentiated Hsp104 variants can display a diminished temperature-sensitive phenotype. (A) W303Δhsp104 yeasts were transformed with the indicated 416GAL-Hsp104 plasmid or empty vector control. Yeasts were grown to saturation in synthetic raffinose medium, serially diluted, and spotted onto SD-Ura or SGal-Ura media and incubated at 30 or 37 °C. Plates were analyzed after 2–3 days of growth. (B) Experiments were carried out as in A to test the putative synergistic variants.