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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Nat Immunol. 2015 Nov 2;17(1):87–94. doi: 10.1038/ni.3310

Fig. 2.

Fig. 2

Signaling properties of synthetic peptide antigens

(a) IL-2 response screen of the CD8+ (black bars) and CD8- (green bars) 42F3 T cells to APCs in the presence of 10μM synthesized peptide from first-generation (left) and second-generation (right) yeast displayed pMHC libraries. Data is represented as mean ± s.e.m. relative A450, normalized to anti-CD3 stimulation condition n=2 of technical replicates. (b) Scatterplots showing a wide range in affinities and activities, but little correlation between the IL-2 EC50 (left) or maximal IL-2 response (Emax) (right, as shown in Supplementary Fig. 2) of CD8+ 42F3 T with affinity of the 42F3-pMHC interactions. pCPE3 is a weak agonist for which EC50 and Emax were not determined, and was therefore omitted from this analysis.