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. Author manuscript; available in PMC: 2017 Jan 28.
Published in final edited form as: Cancer Lett. 2015 Nov 3;370(2):260–267. doi: 10.1016/j.canlet.2015.10.028

Fig. 2.

Fig. 2

PAK4 induces expression of stemness-associated markers in PC cells. (A) PAK4-expressing and silenced cells were incubated with fluorochrome-conjugated anti-CD24, -CD44 and -EpCAM antibodies for 15 min on ice, followed by washing with PBS and subjected to FACS analysis. The data are presented as geometric mean of fluorescence intensity (MFI). The expression of other stemness associated markers was examined (B) at mRNA level by quantitative RT-PCR (mean ± SD; n = 3; *p < 0.05) and (C) protein level by immunoblot assay. β-actin was used as an internal control.