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. Author manuscript; available in PMC: 2017 Jan 28.
Published in final edited form as: Cancer Lett. 2015 Nov 3;370(2):260–267. doi: 10.1016/j.canlet.2015.10.028

Fig. 3.

Fig. 3

PAK4 facilitates sphere formation and drug resistance in PC cells. PAK4 expressing or silenced MiaPaCa and T3M4 cells (1 × 103) were seeded in ultra-low attachment plate and allowed to grow for 10 days in stem cell culture medium. (A) Images were taken under light microscope at 200× magnification and the number of spheres (size > 50 μm) was counted. Error bars represent the mean ± SD; n = 3; *p < 0.05. (B) PAK4 expressing or silenced PC cells were treated with indicated doses of gemcitabine (0–10 μM) for 72 h. Cell viability was analyzed by WST-1 assay. OD value of untreated cells was taken as 100% viable. Data represent the mean ± SD; n = 3; *p < 0.01. (C) Representative images of colonies formed by PAK4-expressing and -silenced cells treated with various concentrations of gemcitabine (0, 0.5, 1.0 and 2.5 μM) for 24 h and further allowed to form colonies for 2 weeks.