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. Author manuscript; available in PMC: 2015 Dec 19.
Published in final edited form as: Science. 2015 Dec 3;350(6267):1548–1551. doi: 10.1126/science.aad8305

Fig. 2. CHMP1B opening, strand structure, and electrostatic surface potentials of the IST1NTD-CHMP1B assembly.

Fig. 2

(A) Superposition of the open and closed CHMP1B conformations. (B) Five interlocked CHMP1B molecules from the inner strand of the filament. (C) “Top-end”, electrostatic surface view of the IST1NTD-CHMP1B tube, highlighting the acidity of the CHMP1B inner strand (including Glu130, Asp131, Asp147, Glu152, Asp155, Glu156, Asp160) and the IST1NTD outer strand (including Asp49, Glu50, Glu57, Glu163, Glu168, Glu178, Asp180, Glu186). (D) “Bottom-end”, electrostatic surface view of the IST1NTD-CHMP1B tube, highlighting the strongly basic characters of the CHMP1B inner strand (including Lys3, Lys87, Lys94, Lys101, Lys107, and Lys114) and the IST1NTD outer strand (including Lys7, Arg10, Lys90, Arg109, Lys118, Lys127, Lys130, Lys134, Arg137). (E) Exterior, electrostatic surface view of the IST1NTD-CHMP1B tube, revealing the modestly basic character of the IST1NTD outer strand. (F) Internal cutaway electrostatic surface view of the IST1NTD-CHMP1B tube, revealing the strongly basic character of the lumenal surface, contributed primarily by basic residues in CHMP1B helix 1 (arrows), including Lys3, Lys13, Arg17, Lys20, Lys21, Lys24, Lys32, and Lys35.