Figure 15.

(A) Anticancer effect of MnTE-2-PyP⁵⁺/Asc on a growth of 4T1 mammary carcinoma in a mouse flank. 10⁶ cells were implanted into the flank of Balb/c mouse. Treatment started 24 hours after the injection of 4T1 tumor cells. MnTE-2-PyP⁵⁺ was administered sc at 0.2 mg/kg/day and ascorbate was given ip at 4g/kg/day throughout the study. 20 mice were used per group. (B) At day 15^th, 95% of mice treated with MnP/Asc have tumors whose volumes were less than 1,500 mm³ (C) ~7-fold higher accumulation of MnTE-2-PyP⁵⁺ was found in tumor than in muscle and was not affected by ascorbate. Of note, a 2^nd study was done where treatment started once tumors on average reached 80 mm³. As similar tumor suppression was observed in either study, early start of treatment when tumor was not established has no additional impact on tumor growth suppression by MnP/Asc.