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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Methods. 2015 Aug 12;91:13–19. doi: 10.1016/j.ymeth.2015.08.008

Figure 3. Optimization of cell lysis conditions.

Figure 3

Infected Huh7 cells were lysed in buffer containing 200 mM KCl, 20 mM HEPES pH 7.2, and the indicated detergents. Insoluble material was cleared from the lysate by centrifugation and total RNA was purified from both soluble and pellet fractions. DENV and nuclear U6 RNAs were quantitated by RT-qPCR. DDM = n-dodecyl β-D-maltoside, NP40 = igepal CA-360, DOC = deoxycholic acid