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. 2015 Dec 6;2015:948540. doi: 10.1155/2015/948540

Figure 1.

Figure 1

Cytokine and interferon secretion from CAL-1 cells upon stimulation with CL264 and 9.2s RNA. CAL-1 cells were seeded into 96-well plates. After overnight resting, cells were stimulated with CL264 or 9.2s RNA (complexed with PLarg) or the combination of both. After incubation as indicated, cell supernatant was analyzed for TNF-α, IL-6, and IFN-β by ELISA. (a) Secretion of TNF-α upon stimulation with CL264 in concentrations from 0.5 to 10 µg/mL and with 9.2s RNA in concentrations from 1 to 2 µg/mL for 6 hrs. (b) (c) (d) Secretion of TNF-α (b), IL-6 (c), and IFN-β (d) upon stimulation with CL264 (5 µg/mL), with 9.2s RNA (2 µg/mL) or the combination of both for 1, 4, and 12 hrs. (e) Secretion of TNF-α upon sequential stimulation. First, cells were incubated with CL264 (5 µg/mL; left hatched bar) or 9.2s RNA (2 µg/mL; right hatched bar) for 2 hrs, followed by 3 wash steps. After subsequent stimulation with the other ligand for additional 6 hrs, cytokine secretion into the supernatant was measured. (f) Secretion of TNF-α upon stimulation with CL264 (5 µg/mL), with 9.2s RNA (2 µg/mL) or with a combination of CL264 and PLarg (2 mg/mL), noncomplexed 9.2s RNA, or nonstimulatory Poly-A RNA (2 µg/mL) for 6 hrs. ∗∗∗ p < 0.005.