Figure 3. kdm5 mutant adults have mitochondrial and metabolic defects.

(A, B) TEM images showing 5K and 10K magnifications of mitochondria within thoracic muscles from wildtype (w¹¹¹⁸) flies. (C, D) TEM images from kdm5^K6801/10424 mutant flies. (E) ATP levels in kdm5^K6801/10424 mutant flies shown as percentage of wildtype and normalized to the total protein levels. * p<0.01. (F) Lactic acid levels in kdm5^K6801/10424 adults relative to w¹¹¹⁸. (G) Quantitation the number of wildtype (w¹¹¹⁸) or kdm5^K6801/10424 flies that climb 7cm in 20 seconds after being tapped to the bottom of the vial. * p<0.001. (H) Citrate levels (ng/μl) in wildtype and kdm5^K6801/10424 mutant flies. * p<0.01. (I) Levels of distinct lipid subtypes in kdm5^K6801/10424 mutant flies relative to wildtype. LysoPC is lysophosphatidylcholine acyls (fatty acids generated as intermediate products), PCaa is phosphatidylcholine acyl molecules and PCae is phosphatidylcholine acyl-alkyl molecules. Numbers (<38 and >38) indicate number of carbon atoms in fatty acid chain. * p<0.01. (J) Ratio of NAD+ to NADH in wildtype (w¹¹¹⁸) and kdm5^K6801/10424 mutant flies. * p<0.001. (K) Levels of superoxide as assayed by MitoSOX in kdm5^K6801/10424 mutant flies relative to wildtype. * p<0.05. (L) Levels of the antioxidant carnosine (pmol/mg tissue) in wildtype and kdm5^K6801/10424 mutant flies. * p≪0.001. (M) Detection of oxidized proteins in wildtype and kdm5^K680/104241 mutant fly heads using oxyblot (2-fold increase).