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. Author manuscript; available in PMC: 2015 Dec 20.
Published in final edited form as: Cell Rep. 2015 Dec 7;13(10):2219–2231. doi: 10.1016/j.celrep.2015.11.007

Figure 4. KDM5-mediated regulation of mitochondrial function genes is independent of its demethylase activity.

Figure 4

(A) Real-time PCR analyses of the genes indicated in demethylase inactive adults (described in (Li et al., 2010); kdm5JmjC*) relative to wildtype (w1118). Each gene was normalized to rp49 and shown as relative expression in kdm5JmjC mutants. * p<0.05. (B, C) TEM images showing 5K magnifications of mitochondria within thoracic muscles from kdm5K6801 mutant flies rescued by a wildtype KDM5 transgene (KDM5WT; B) and kdm5JmjC mutant flies (C). (D) ChIP-PCR showing levels of histone H3 acetylation using a pan-acetyl antibody in wildtype and kdm5K6801/10424 flies. IgG is a negative control. ChIP-PCR fragments are indicated in Figure 2B. * p<0.05.