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. Author manuscript; available in PMC: 2016 Nov 18.
Published in final edited form as: Neuron. 2015 Nov 18;88(4):667–677. doi: 10.1016/j.neuron.2015.10.038

Figure 2. HD-RAN Proteins in Striatum.

Figure 2

(A) Control and HD brain showing striatal sub-regions with summary of staining.

(B) Quantification of IHC-positive cells for α-Gln, α-RAN, and IBA1 staining ± SEM in caudate nucleus.

(C–I) IHC of striatal sub-sections from HD brains using (C) α-Gln antibody (1C2), ([D]–[G]) α-RAN antibodies, (H) α-IBA1 for microglia, and (I) α-active caspase-3 for cell death. (A)–(I): red, positive staining; blue, nuclear counterstain.

(J) Double staining for RAN protein cocktail using mixture of all four anti-RAN antibodies (brown) plus anti-IBA1 antibody to label microglia with quantitation (± SEM) of single-, double-labeled, and IBA1 cells that are in close proximity to RAN-positive cells.

(K) Double staining for RAN protein cocktail using mixture of all anti-RAN antibodies (brown) plus anti-caspase-3 antibody to label apoptotic cells with quantitation of the percent active Caspase-3-positive cells that are also positive for RAN proteins. See also Figure S2. Cau-WMB, caudate white matter bundles; Int-Cap, internal capsule; Put-WMB, putamen white matter bundles.