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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Cancer Res. 2015 Aug 19;13(12):1554–1566. doi: 10.1158/1541-7786.MCR-15-0240

Fig. 6.

Fig. 6

Knockdown of Srx stimulates lysine acetylation in EGFR specifically on K1037 as identified by LC-ESI-MS/MS. (A) Knockdown of Srx in HCT116 cells increases the total levels of lysine acetylation in EGFR. (B) Quantitative analysis of data in (A). (C) Ectopic expression of FlagSrx in HT-29 cells decreases the levels of lysine acetylation in EGFR. (D) Quantitative analysis of data in (C). (E) Identification of K1037 acetylation by MS in HCT116 ShSrx and HT29 cells. The upper panel shows a MS-spectrum from peptide containing acetylated K1037 and the bottom panel shows a MS-spectrum from non-acetylated intact peptide. *Compared with ShNT or cells expressing empty vector, p<0.05 (n = 3).