Table 2.
Peripheral B cells in genetically γδ T cell-deficient mice
| Percentage (Absolute cell #) |
B6 (n=8) |
δ−/− (n=4) |
Vγ1−/− (n=5) |
Vγ4−/−/6−/− (n=8) |
Vγ4−/−/6−/−/IL4−/− (n=7) |
|
|---|---|---|---|---|---|---|
| Lymph node | ||||||
| B/Lym (%) | 23.9±9.8 | 24.9±0.9 | 15.0±2.2 | 8.5±3.0§ | 27.5±7.9 | |
| (B cells (x106)) | (1.9±0.7) | (2.9±0.3)* | (1.6±0.5) | (0.4±0.2)¶ | (2.8±1.0) | |
| B2 B/B (%) | 94.0±1.6 | 92.9±0.9 | 62.3±6.2¶ | 79.9±4.5¶ | 91.5±2.3 | |
| (B2 B cells (x106)) | (1.7±0.6) | (2.7±0.3)* | (1.0±0.4)¶ | (0.3±0.1)¶ | (2.6±0.8) | |
| B1 B/B (%) | 2.1±0.6 | 3.0±0.4 | 6.3±1.9¶ | 13.5±4.8¶ | 3.3±0.8 | |
| (B1 B cells (x103)) | (54.7±39.6) | (92.9±21.6) | (100.2±47.5) | (43.8±22.5) | (97.2±52.2) | |
| Peritoneal cavity | ||||||
| B/Live (%) | 25.1±1.6 | 21.4±4.0 | 32.0±7.5* | 13.3±5.3¶ | 26.0±9.5 | |
| (B cells (x103)) | (394.0±148.6) | (530.2±31.8*) | (1027.5±465.3¶) | (227.2±153.3) | (566.7±243.8) | |
| B2 B/B (%) | 18.5±4.1 | 20.7±7.0 | 8.2±2.4¶ | 21.9±7.3 | 21.8±9.1 | |
| (B2 B cells (x103)) | (57.4±17.6) | (54.3±4.8) | (84.6±40.2) | (41.0±25.9) | (122.5±87.4) | |
| B1 B/B (%) | 67.5±5.6 | 66.2±7.6 | 70.8±2.7 | 60.8±6.8 | 57.8±17.9 | |
| (B1 B cells (x103)) | (269.5±112.2) | (346.5±31.2) | (727.4±334.2§) | (143.7±104.84) | (384.1±176.0) | |
| B1a B/B1 B (%) | 83.5±2.3 | 83.8±2.8 | 88.0±1.8 | 20.5±7.1¶ | 74.8±6.1* | |
| (B1a B cells (x103)) | (226.7±98.0) | (290.9±35.7) | (642.8±305.9§) | (34.4±32.9¶) | (273.9±137.3) | |
| B1b B/B1 B (%) | 16.2±2.3 | 15.9±2.7 | 11.5±1.7 | 79.1±6.8¶ | 25.0±6.2* | |
| (B1b B cells (x103)) | (42.0±14.7) | (54.3±4.8) | (81.5±31.6*) | (109.0±73.8*) | (109.8±57.4*) | |
| B1a/B1b | 5.3±0.9 | 5.4±1.0 | 7.8±1.4§ | 0.3±0.1¶ | 3.2±1.1§ | |
| Blood | ||||||
| B/Lym (%) | 50.9±10.4 | 66.9±4.1* | ND | 48.1±5.3 | 68.1±11.0 | |
| B2 B/B (%) | 83.1±3.2 | 85.3±1.9 | ND | 63.1±2.1¶ | 89.6±2.3 | |
| B1 B/B (%) | 2.2±0.6 | 2.0±0.4 | ND | 3.7±0.2¶ | 1.4±0.5 | |
| B1a B/B1 B (%) | 73.3±6.5 | 70.5±7.1 | ND | 33.6±7.0¶ | 67.8±3.5 | |
| B1b B/B1 B (%) | 26.0±6.6 | 29.5±7.1 | ND | 65.4±7.0¶ | 32.3±3.5 | |
| B1a/B1b | 3.0±0.9 | 2.5±0.8 | ND | 0.5±0.2¶ | 2.1±0.3 | |
| Spleen | ||||||
| Total B cells | ||||||
| B/Lym (%) | 58.0±3.0 | 52.5±8.7 | 38.3±10.6§ | 29.3±1.9¶ | 52.0±2.7* | |
| (B cells) | (45.3±10.2) | (40.1±8.7) | (41.1±9.8) | (11.2±2.4)¶ | (45.6±21.3) | |
| Mature B cells | ||||||
| Mature B/Lym (%) | 46.8±2.0 | 43.9±9.7 | 32.3±8.8§ | 25.7±1.8¶ | 46.9±4.5 | |
| (Mature B cells) | (36.3±6.7) | (33.6±9.3) | (34.6±8.2) | (9.7±1.8)¶ | (41.4±20.8) | |
| MZB/Mature B (%) | 4.3±0.8 | 3.8±1.1 | 9.5±1.2¶ | 0.6±0.2¶ | 4.7±1.8 | |
| (MZB) | (1.6±0.4) | (1.3±0.6) | (3.3±0.9)¶ | (0.1±0.0) ¶ | (1.7±0.2) | |
| FOB/Mature B (%) | 84.0±1.4 | 81.2±3.6 | 74.9±9.2* | 62.7±6.5¶ | 80.0±2.2 | |
| (FOB) | (30.5±5.9) | (27.4±8.1) | (25.9±7.2) | (6.1±1.5)¶ | (32.9±15.8) | |
| NewB/Mature B (%) | 8.3±1.3 | 11.2±4.4 | 10.5±4.0 | 32.2±8.9¶ | 11.4±3.6 | |
| (NewB) | (3.0±0.6) | (3.6±0.9) | (4.8±3.3) | (3.1±0.9) | (5.3±4.3) | |
| Immature B cells | ||||||
| Immature B/Lym (%) | 9.5±1.1 | 8.6±2.4 | 6.1±2.2§ | 3.9±0.4¶ | 6.1±0.9¶ | |
| (Immature B) | (8.1±1.2) | (3.4±0.9) | (2.6±1.3) | (2.1±1.1)¶ | (5.7±1.0)* | |
| T1/Imm (%) | 27.4±1.2 | 25.4±1.0 | 27.6±0.9 | 48.0±1.2¶ | 25.8±3.3¶ | |
| (T1) | (2.1±0.2) | (1.5±0.3) | (2.0±0.3) | (1.0±0.1)¶ | (1.6±0.5) | |
| T2+T3/Imm (%) | 59.9±1.5 | 59.8±1.6 | 61.1±1.2 | 33.4±1.1¶ | 55.9±3.5 | |
| (T2+T3) | (5.0±0.8) | (3.3±0.6)§ | (4.3±0.5) | (0.7±0.1)¶ | (3.0±0.5) | |
| B2 cells | ||||||
| B2/B (%) | 76.9±3.0 | 78.9±0.4 | 32.5±2.8¶ | 58.7±5.2¶ | 80.2±0.5 | |
| (B2) | (36.5±10.4) | (31.6±8.0) | (13.2±3.6)¶ | (5.9±1.1)¶ | (37.8±18.3) | |
| B1 cells | ||||||
| B1/B (%) | 2.1±0.3 | 2.6±0.9 | 2.8±0.7 | 10.8±3.3¶ | 3.2±1.9 | |
| (B1) | (0.8±0.2) | (1.2±0.6) | (1.1±0.4) | (0.9±0.3) | (1.3±1.2) | |
*
p<0.05
§
p<0.01
¶
p<0.001; ND, not determined
Peripheral B cells in various γδ T cell knockout mice. B cells in lymph nodes, peritoneal cavity, blood and spleen were identified based on their surface phenotype as described in Figures 1 and 3, and enumerated in individual mice. Absolute numbers (in parentheses) and relative frequencies are shown. n = 4–8 mice per group, p values, as indicated in the table.