Table 3.
B cells in bone marrow from genetically γδ T cell-deficient mice
| Percentage % (Absolute cell # x106) |
B6 (n=13) |
δ−/− (n=6) |
Vγ1−/− (n=5) |
Vγ4−/−/6−/− (n=4) |
Vγ4−/−/6−/−/IL4−/− (n=5) |
|---|---|---|---|---|---|
| B cells/live cells | 29.0±3.9 | 29.7±4.0 | 23.5±3.6* | 24.4±1.7§ | 27.7±3.6 |
| (B cells) | (6.4±2.1) | (6.7±2.2) | (4.6±1.7) | (4.9±0.9) | (6.7±2.1) |
| Fr’A/(A–C) | 51.0±5.1 | 47.3±6.0 | 40.5±7.0* | 56.1±2.2 | 53.5±7.0 |
| (Fr’A) | (0.4±0.1) | (0.4±0.1) | (0.3±0.1)§ | (0.5±0.2) | (0.3±0.1) |
| Fr’B/(A–C) | 19.3±1.6 | 20.2±3.0 | 30.4±9.3 | 18.2±5.1 | 18.4±4.0 |
| (Fr’B) | (0.2±0.1) | (0.2±0.0) | (0.2±0.2) | (0.2±0.0) | (0.1±0.0) |
| Fr’C/(A–C) | 26.2±4.3 | 28.7±3.6 | 24.2±3.6 | 25.5±3.5 | 23.6±5.0 |
| (Fr’C) | (0.2±0.1) | (0.2±0.0) | (0.2±0.1) | (0.3±0.1) | (0.1±0.0) |
| Fr’D/(D–F) | 59.5±4.1 | 59.7 ±3.8 | 47.8±5.5§ | 72.4±9.8* | 64.4±4.7 |
| (Fr’D) | (3.3±1.2) | (4.0±1.1) | (1.9±0.9)* | (3.1±0.6) | (3.8±1.2) |
| Fr’E/(D–F) | 15.9±2.4 | 14.1±2.0 | 15.5±1.6 | 17.7±8.0 | 14.5±2.5 |
| (Fr’E) | (0.9±0.3) | (1.1±0.3) | (0.6±0.3) | (0.7±0.3) | (0.9±0.3) |
| Fr’E’/(D–F) | 4.2±0.6 | 4.1±0.6 | 5.1±0.3§ | 4.1±0.9 | 3.4±1.1 |
| (Fr’E’) | (0.2±0.1) | (0.2±0.1) | (0.2±0.1) | (0.2±0.0)* | (0.2±0.1) |
| Fr’F/(D–F) | 16.3±4.7 | 18.6±2.4 | 20.9±6.4 | 2.1±1.5¶ | 14.4±1.8 |
| (Fr’F) | (0.9±0.4) | (1.1±0.3)* | (0.8±0.3) | (0.1±0.1)¶ | (0.8±0.2) |
p<0.05
p<0.01
p<0.001
Bone marrow B cells in various γδ T cell knockout mice. B cell types were identified based on their surface phenotype as in Figure 2. Following a scheme first described by Hardy et al. (92), we divided B220posCD43pos early stage bone marrow B cells into developmentally consecutive fractions A-C based on their expression of CD24 and BP1. These cells represent pro-B and early pre-B cells. Later stage B220posCD43neg B cells, which include those that returned from the periphery, were divided into increasingly mature fractions D (pre-B cells), E and F (B cells), based on their expression of IgM and IgD. Cells contained within these fractions were enumerated in individual mice. Absolute numbers (in parentheses) and relative frequencies are shown. n = 4–13 mice per group, p values, as indicated in the table.