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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Immunol. 2015 Nov 18;196(1):336–344. doi: 10.4049/jimmunol.1502037

Figure 1. Effect of neutrophil calprotectin on A. fumigatus corneal infection.

Figure 1

A. C57BL/6 or S100A9−/− mice were infected intrastromally with 50,000 A. fumigatus dsRed conidia (A-D) and corneas were imaged at 24 and 48h pi. B. Fungal burden measured by dsRed fluorimetry at 24 and 48h pi. C. CFU from whole eye homogenates measured at 48h pi. D. Neutrophil number in infected corneas measured as total NIMP-R14+ cells in corneal cell suspensions at 24 and 48h pi by flow cytometry. E. PASH and IHC for neutrophils (NIMP-R14), S100A8 and S100A9 in adjacent corneal sections from A. fumigatus infected C57BL/6 mice at 24 and 48h pi (400X). F. S100A8 and S100A9 expression by ELISA in naïve or A. fumigatus infected corneas of C57BL/6 or CD18−/− mice. G. NIMP-R14 staining in S100A8 and S100A9-positive cells from A. fumigatus infected corneas of C57BL/6 mice at 24h pi. H-I. S100A9−/− mice were infected intrastromally with 50,000 A. fumigatus dsRed conidia and PBS or recombinant calprotectin (1 µg) was injected subconjunctivally at the time of infection. H. Representative images of A. fumigatus dsRed in infected corneas at 24 hrs pi. I. Fungal burden measured by dsRed fluorimetry at 24h pi. Experiments were repeated three times with 3–5 mice per group, A Mann-Whitney U test was used to determine significance. All data show mean +/− SEM. See also Figure S1.