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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Immunol. 2015 Nov 18;196(1):336–344. doi: 10.4049/jimmunol.1502037

Figure 2. Effect of neutrophil calprotectin on A. fumigatus hyphal growth in vitro.

Figure 2

Peritoneal neutrophils or neutrophil lysates from C57BL/6 or S100A9−/− mice were incubated 16h with A. fumigatus hyphae (A-B), and hyphal growth was assessed by calcofluor staining A. Representative images of hyphae (200X); B-C. Quantification of hyphal growth in the presence of neutrophils or neutrophil lysates. % Fungal mass was calculated by fluorimetry of calcofluor and shown as percent of total hyphae when grown in media alone (experimental/control × 100). Representative In vitro experiments with 3–6 technical replicates per condition, significance was determined by student’s t-test. All data show mean +/− SEM. See also Figure S1.