Skip to main content
. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Immunol. 2015 Nov 18;196(1):336–344. doi: 10.4049/jimmunol.1502037

Figure 4. ZafA mediated zinc uptake in A. fumigatus virulence and susceptibility to calprotectin.

Figure 4

WT or ΔzafAA. fumigatus hyphae were incubated with peritoneal neutrophils from C57BL/6 or S100A9−/− mice or recombinant calprotectin for 16h (A-B). Fungal growth was assessed by calcofluor staining and % Fungal mass was calculated by fluorimetry of calcofluor and shown as percent of total hyphae when grown in media alone (experimental/control × 100). A. Growth of hyphae incubated with C57BL/6 or S100A9−/− neutrophils (0.5 × 105/well). B. Growth of hyphae incubated with increasing doses of CP. Representative In vitro experiments with 3–6 technical replicates per condition, significance was determined by student’s t-test. C57BL/6 mice were infected intrastromally with 50,000 WT or ΔzafA A. fumigatus conidia (C-D). C. Fungal growth assessed in infected corneal sections by GMS stain (hyphae appear black) at 48h pi (400X). D. CFU of WT vs. ΔzafA from whole eye homogenates measured at 48h pi. E-F. Neutrophil infiltration in C57BL/6 mice infected with WT or ΔzafAA. fumigatus assessed by IHC for NIMP-R14 in corneal sections and quantified using Metamorph (400x). In vivo experiment was repeated three times with at least 3 mice per group. Significance was assess by Mann-Whitney U test. All data show mean +/− SEM. See also Figure S2.