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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Immunol. 2015 Nov 16;196(1):357–364. doi: 10.4049/jimmunol.1501494

Figure 2.

Figure 2

Differential kinetics and sensitivity of antigen-specific CD4+ cell priming in M. bovis BCG and M. tuberculosis infection. Proliferation of adoptively transferred CFSE-labeled Ag85B peptide 25-specific TCR transgenic (P25TCR-Tg) CD4+ T cells in the lung-draining lymph node was assayed at selected times after aerosol M. bovis BCG or M. tuberculosis infection. Representative CFSE dilution profiles of P25TCR-Tg CD4+ T cells after aerosol infection with (A) M. bovis BCG or (B) M. tuberculosis. (C) Total number of CFSEdilute P25TCR-Tg cells CD4+ T cells in lung-draining lymph nodes after infection with M. bovis BCG or M. tuberculosis, calculated from frequencies determined by flow cytometry. (D) Quantitation of live M. bovis BCG or M. tuberculosis H37Rv in lung-draining lymph nodes after aerosol infection. The dashed line depicts the limit of detection for viable bacteria (6 cfu/lymph node). Data are expressed as mean ± SEM and are representative of 2 experiments where n = 4 mice per time point for each bacterial infection. Student t test was used to compare BCG and M. tuberculosis samples at each time point: *, P<0.05; **, P<0.01; ***, P<0.005; ****, P<0.0001.