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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Immunol. 2015 Nov 23;196(1):428–436. doi: 10.4049/jimmunol.1501688

Figure 6. TRPV4 mediates LPS-stimulated macrophage phagocytosis of IgG-coated latex beads in vivo.

Figure 6

WT and TRPV4 KO C57BL/6 were treated with IT LPS (3 µg/g) for 16 h followed by intratracheal (IT) IgG-coated latex beads for 6 h. Cell phagocytic analysis was performed on the BAL by microscopic analysis of cytospin preparations. (A) Representative confocal images of WT and TRPV4 KO mice given IT saline (n = 2) or LPS (n = 5) followed by IgG-coated latex beads (white arrow heads) - Green-beads, Blue-nuclei (PMN: multilobular nucleus, macrophage: single concentric nucleus), Red-CD45 to show membrane (All panels, 40X Orig. Mag.). (B) LPS treated WT mice had increased macrophage phagocytosis of IgG-coated latex beads compared to the LPS treated TRPV4 KO. The number of beads per macrophage were quantified from panel A (*,+p < 0.05). + denotes the increase in LPS vs UT, * denotes difference in LPS response between KO and WT.